Measuring leaf sizes in ImageJ

Cheatsheet

Author

Dylan de Domizio

Published

August 4, 2026

This work was developed using resources that are available under a Creative Commons Attribution 4.0 International License, made available on the SOLES Open Educational Resources repository by the School of Life and Environmental Sciences, The University of Sydney.

NoteAssumed knowledge

About

This cheatsheet shows how to measure leaf sizes in ImageJ using an 8-bit thresholding workflow. The video was created by Dylan de Domizio, a BEDA student who has kindly agreed to share his workflow for future students.

The workflow converts the image to 8-bit greyscale, applies a threshold to separate the leaf from the background, and measures the resulting leaf area. It also handles common issues such as labels or writing on the leaf.

For now, it is a single video tutorial, but a written version will be added to this section in the near future.

Video tutorial

NoteAttribution

“ImageJ Tutorial - Measuring Leaf Sizes” by Dylan de Domizio, released under a Creative Commons Attribution 4.0 International (CC-BY 4.0) licence by agreement with the author. View the original video on YouTube.